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高温胁迫下外源钙对菊花叶片光合机构与活性氧清除酶系统的影响

Effects of exogenous Ca2+on leaf photosynthetic apparatus and active oxygen scavenging enzyme system of chrysanthemum under high temperature stress

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【作者】 孙宪芝郭先锋郑成淑王文莉梁芳

【Author】 SUN Xian-zhi1,GUO Xian-feng2,ZHENG Cheng-shu1,WANG Wen-li1,LIANG Fang1(1State Key Laboratory of Crop Biology,College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an 271018,Shandong China;2College of Forestry,Shandong Agricultural University,Tai’an 271018,Shandong,China)

【机构】 山东农业大学园艺科学与工程学院作物生物学国家重点实验室山东农业大学林学院

【摘要】 以切花菊品种‘神马’为材料,研究了高温胁迫下外源Ca2+对菊花光合系统及活性氧清除酶系统的影响,探讨了Ca2+可能的作用机制.结果表明:高温胁迫下外源Ca2+抑制了净光合速率(Pn)与实际量子效率(ΦPSⅡ)的大幅度降低,24h后,二者分别比对照高出31.11%与21.88%,而初始荧光(Fo)比对照降低了13.19%;Ca2+明显激活了高温胁迫下叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活性,活性氧得到及时清除,24h后,膜脂过氧化产物丙二醛(MDA)的积累量与相对电导率(REC)分别比对照降低29.20%与35.81%,缓解了短期高温胁迫对菊花光合系统的破坏.

【Abstract】 Taking cut flower chrysanthemum ’Jinba’ as test material,the effects of exogenous Ca2+ on its photosynthetic system and antioxidant enzyme activities under high temperature stress were investigated,with the possible action mechanisms of Ca2+ discussed.The results showed that under high temperature stress,Ca2+ addition greatly inhibited the net photosynthesis rate(Pn)and quantum yield of PSⅡ electron transport(ΦPSⅡ).After 24 h treatment,the Pn and ΦPSⅡ were increased by 31.11% and 21.88%,respectively,and the initial fluorescence(Fo)decreased by 13.19%,compare with the control.Ca2+ addition also greatly enhanced the activities of SOD,POD and CAT,and thus,the active oxygen was scavenged timely.After 24 h treatment,the MDA accumulation and REC were 29.20% and 35.81% lower than the control,respectively.In conclusion,Ca2+ addition could efficiently protect chrysanthemum leaves from the damage in photosynthetic apparatus under short-term high temperature stress.

【基金】 教育部留学回国人员项目(33206);国家“十一五”科技支撑计划资助项目(2006BAD10B07)
  • 【文献出处】 应用生态学报 ,Chinese Journal of Applied Ecology , 编辑部邮箱 ,2008年09期
  • 【分类号】S682.11
  • 【被引频次】98
  • 【下载频次】769
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