【摘要】 采用水培方法研究了LaCl3对硝酸盐胁迫下黄瓜幼苗生长及叶片抗氧化酶活性的影响.结果表明:硝酸盐胁迫显著抑制了黄瓜幼苗的生长,尤其是地上部生长.在处理7d时,与对照相比,硝酸盐胁迫下的黄瓜单株地上部鲜质量降低了12.77g,叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性升高,而抗坏血酸过氧化物酶(APX)、脱氢抗坏血酸还原酶(DHAR)和谷胱甘肽还原酶(GR)活性显著降低.外加低浓度(0.05mmol.L-1)LaCl3可以显著提高硝酸盐胁迫下黄瓜幼苗单株鲜质量,比硝酸盐胁迫下提高了35%左右,还提高了叶片热稳定蛋白含量及SOD、POD、CAT、APX、DHAR、GR活性,降低了电解质渗漏率及丙二醛(MDA)含量,在一定程度上缓解了硝酸盐对黄瓜幼苗生长的抑制作用.但外加高浓度LaCl3(0.5mmol.L-1)长期处理对黄瓜硝酸盐胁迫的缓解效果并不明显.因此外加一定浓度的LaCl3可通过提高黄瓜抗氧化酶活性及热稳定蛋白含量来缓解硝酸盐胁迫.更多还原

【Abstract】 The study with water culture showed that nitrate stress had significant inhibitory effects on the plant growth,especially the shoot growth of cucumber seedlings.On the 7th day under nitrate stress,the shoot fresh mass per plant decreased by 12.77 g,leaf SOD,POD and CAT activities increased,while leaf APX,DHAR and GR activities decreased significantly,compared with the control.The addition of low concentration(0.05 mmol·L-1)LaCl3 increased the shoot fresh mass per plant by 35%,enhanced the leaf thermally stable protein content and SOD,POD,CAT,APX,DHAR and GR activities,and decreased the leaf MDA content and electrolytic leakage,resulting in a definite alleviation of the inhibitory effects of nitrate.However,an addition of high concentration(0.5 mmol·L-1)LaCl3 had no obvious alleviation effect.It was concluded that the addition of certain concentration LaCl3 could alleviate the nitrate stress on cucumber via increasing anti-oxidative enzymes activities and thermally stable protein content.更多还原