【摘要】 采用分子印迹技术合成了以依诺沙星为模板,以甲基丙烯酸和4-乙烯基吡啶同时为功能单体的分子印迹聚合物。运用平衡结合实验研究了聚合物的吸附特性和选择识别能力。Scatchard分析表明,该分子印迹聚合物在识别依诺沙星分子的过程中存在2类不同的结合位点,高亲和力结合位点的离解常数Kd1=0.22 mmol/L,最大表观结合常数Qmax1=52.2μmol/g;低亲和力结合位点的离解常数Kd2=1.23 mmol/L,最大表观结合常数Qmax2=150.5μmol/g。结合底物的实验结果表明,依诺沙星分子印迹聚合物对依诺沙星呈现较高的吸附性能和选择识别特性。将该印迹聚合物作为固相萃取剂,在人血清依诺沙星的分析中对样品进行了有效的净化和富集。更多还原

【Abstract】 With enoxacin as template molecule,methacrylic acid and 4-rinylpyridine as functional monomers,a molecularly imprinted polymer was prepared via a molecular imprinting technique.The molecularly imprinted polymer was investigated in equilibrium binding experiment to evaluate its adsorption property and selective recognition.Scatchard analysis showed that two classes of binding sites were formed in the enoxacin imprinted polymer under the studied concentrations.The dissociation constant(Kd1) and the apparent maximum binding capacity(Qmax) were Kd1=0.22 mmol/L,Qmax1=52.2 μmol/g for high affinity binding sites and Kd2=1.23 mmol/L,Qmax2=150.5 μmol/g for low affinity binding sites,respectively.The experiments of binding different substrates indicated that the imprinted polymer possessed a high selectivity and a high recognition ability for enoxacin.When it was used as a solid phase extraction sorbent,the imprined polymer effectively completed the clean-up and enrichment of the sample in the analysis of enoxacin from human serum.更多还原