【摘要】 目的建立反相高效液相色谱(RP-HPLC)法测定强力天麻杜仲胶囊中新乌头碱和次乌头碱的含量。方法色谱柱为Agilent Zorbax Stable BondC18柱(4.6mm×250mm,5μm),流动相为用磷酸调pH=3的0.04mol/L三乙胺-乙腈(65∶35),流速1.0mL/min,检测波长235nm。结果新乌头碱、次乌头碱能良好分离,进样量分别在0.0468～1.17μg和0.0506～1.265μg范围内与峰面积线性关系良好,平均回收率分别为98.94%和98.98%,RSD分别为1.70%和1.56%(n=6)。结论方法简便、精确、专属性强,可作为强力天麻杜仲胶囊中乌头碱类成分的质量控制方法。更多还原

【Abstract】 Objective To estahlish a RP-HPLC method of determining mesaconitine,hypaconitine in Qiangli Tianmaduzhong Capsules.Methods RP-HPLC was applied to determine mesaconitine,hypaconitine in Qiangli Tianmaduzhong Capsules.HPLC conditions were as follows:Agilent Zorbax StableBond C18 column(250 mm×4.6 mm,5 μm)was used,with acetonitrile-0.04 mol/L triethylamine adjusting pH=3 with H3PO4(65:35)as the mobile phase.The flow rate was 1.0 mL/min.The detection wavelenth was set at 235 nm.Results mesaconitine,hypaconitine derivatives were separated well.The linear range for mesaconitine and hypaconitine were 0.046 8-1.17 μg and 0.050 6-1.265 μg,respectively.Their average recovery rates were 98.94%with RSD of 1.70%and 98.98%with RSD of 1.56%,respectively.Conclusion The method is simple and accurate with strong specificity.It can be used to control the quality of mesaconitine,hypaconitine in Qiangli Tianmaduzhong Capsules.更多还原