【摘要】 目的检测大青叶提取物体外抗单纯疱疹病毒Ⅱ型(HSV-Ⅱ)的作用。方法以Vero细胞为宿主细胞,阿昔洛韦为阳性对照药物,通过观察细胞病变效应(CPE)和改良MTT比色法来测定药物的细胞毒性、药物对HSV-Ⅱ的直接灭活作用,以及药物抗HSV-Ⅱ对细胞的吸附和对HSV-Ⅱ在细胞内复制增殖的抑制,算出药物抗HSV-Ⅱ的治疗指数。结果大青叶提取物在体外对HSV-Ⅱ无直接灭活作用,也无抗HSV-Ⅱ吸附细胞的作用,但能抑制HSV-Ⅱ在细胞内的复制增殖,其治疗指数达3.56。结论大青叶提取物在体外有一定的抗HSV-Ⅱ感染效果,主要是通过抑制病毒在细胞内的复制增殖而发挥作用。更多还原

【Abstract】 Objective To study effects of the extract from indigowoad leaf on herpes simplex virus(HSV)-Ⅱin vitro. Methods Using Vero cell as host cells and acyclovir as positive control,the cytotoxicities on cells by the herbs,the direct virus destroying and inhibition on virus proliferation were investigated by adopting MTT assay and observing cytopathic effect(CPE). Results It was showed that the herbs had no direct inactivation on HSV-Ⅱin vitro,no inhibition on virus absorption either.However, the herbs could inhibit HSV-Ⅱ biosynthesis and multiplication.The treatment index(TI) was 3.56. Conclusion The extracts from indigowoad leaf show some anti-HSV-Ⅱ effects in vitro by suppressing virus replication in cells.更多还原