【摘要】 目的探讨甲醛致生物机体DNA-蛋白质交联(DNA-Protein crosslinks,DPC)作用。方法研究以Wistar大鼠肝组织为实验材料进行体内和体外实验,采用KCl-SDS沉淀法来检测甲醛染毒后大鼠肝细胞DNA-蛋白质交联的含量。结果低浓度的气态甲醛(0.5 mg/m3)不能引起DNA-蛋白质的交联,较高浓度的甲醛(1.0mg/m3,3.0mg/m3)可以诱导明显的DNA-蛋白质交联作用(P<0.01);体外实验结果显示:经低浓度甲醛(12.5μmol/L)处理1h后,大鼠肝细胞内的DPC系数稍有变化,但与空白对照组相比无显著差异,而随着甲醛浓度上升,细胞中的DPC含量出现了显著性上升。结论体内和体外实验表明:低浓度的甲醛不能引起DNA-蛋白质的交联,而较高浓度的甲醛可以引起明显的DNA-蛋白质的交联作用。更多还原

【Abstract】 Objective To explore the(DNA-protein crosslinks,DPC) damages of rats induced by formaldehyde.Methods The liver cells of rats were used as experimental materials in vivo and in vitro respectively,and KCl-SDS assay was applied to determine the amount of DPC.Results The results in vivo showed that gaseous formaldehyde could cause DPC at high concentrations(1.0mg/m3,3.0mg/m3,P<0.01),but not happened the case at low concentrations(0.5mg/m3).Meanwhile,the results in vitro showed that liquid formaldehyde at low concentration(12.5μmol/L) resulted in an insignificant increase in DPC content compared with control groups.But as formaldehyde concentration ascends,DPC could be formed significantly.Conclusion These results suggested that formaldehyde can induce DPC at relatively high concentration but not at low.更多还原