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毛细管电泳及液相色谱法研究黄连黄芩配伍过程化学成分的变化
Studies on change of chemical composition in Coptis-Scute herb couple by using HPCE and HPLC
【摘要】 对黄连与黄芩的不同比例配伍进行定量研究。黄连与黄芩按6∶1、6∶4、6∶7、6∶10、6∶13比例配伍,采用高效毛细管电泳法测定了黄连单煎液、黄连黄芩共煎液中生物碱的含量;采用高效液相色谱法测定了黄芩单煎液、黄连黄芩共煎液中黄酮的含量。结果显示,黄连黄芩共煎产生沉淀,使有效成分的含量降低。
【Abstract】 Quantitative analysis on different proportions of couple of Coptis and Scute. The proportions of Coptis and Scute couple were 6 ∶ 1, 6 ∶ 4, 6 ∶ 7, 6 ∶ 10, 6 ∶ 13, separately. The contents of alkaloids in Coptis and decoction of Coptis and Scute were determined by HPCE and the contents of flavones in Scute and decoction of Coptis and Scute were determined by HPLC, separately. Precipitates were generated after the couple of Coptis and Scute, which results the decrease of contents of effective ingredients.
【基金】 国家自然科学基金资助项目(30472134,30672600);中国科学院知识创新工程重要方向项目资助(KGCX2-SW-213-06);吉林省科技发展计划项目资助(20060902);长春市科技发展计划项目资助(04-02GG233)
- 【文献出处】 药学学报 ,Acta Pharmaceutica Sinica , 编辑部邮箱 ,2008年02期
- 【分类号】R284
- 【被引频次】26
- 【下载频次】726