【摘要】 [目的]研究阻抑动力学光度法测定痕量EDTA的新体系。[方法]在硫酸介质中,Fe(Ⅲ)催化KIO4氧化二溴硝基偶氮胂的反应可被微量EDTA有效地阻抑,被过量EDTA有效的终止,据此建立了测定痕量EDTA的动力学光度法,用固定时间法在510 nm波长处监测阻抑反应。[结果]方法的线性关系良好,线性范围为0~50.0μg/50 ml,检出限为2.0×10-6mg/mL,标准加入回收率为97.0%~102.0%。[结论]该方法用于罐头等食品中EDTA的测定,获得满意结果。更多还原

【Abstract】 [Objective]To study a new system of determining trace EDTA by inhibition kinetic-spectrophotometry.[Methods]In the medium of H2SO4 solution,the catalytic action of Fe(Ⅲ)on the oxidation of Dibromo-nitro Arsenazo by KIO4 was effectively inhibited by EDTA.The reaction was effectively stopped by superfluous EDTA.The decrease in absorbance was measured at 510 nm at fixed time intervals.[Results]The linear determination range is between 0 and 50.0 μg/50 ml of EDTA.A detection limit of 2.0×10-6 mg/mL was achieved.The recoveries were 97.0%~102.0%.[Conclusion]The method can be applied in the determination of trace EDTA in canned food with satisfactory results.更多还原