【摘要】 目的:建立高效液相色谱手性添加剂法测定特布他林对映体血药浓度。方法:用Waters AtlantisC18色谱柱(4.6 mm×150 mm,5μm);流动相为水-乙腈(95∶5),其中含8.4 mmol.L-1β-CD(作为手性流动相添加剂)、0.05 mmol.L-1醋酸铵且pH3.0;流速为0.6 mL.min-1;荧光检测,Ex=280 nm,Em=320 nm;进样量5μL;柱温10℃。结果:特布他林两对映体在0.25~25 mg.L-1的浓度范围内线性良好。结论:该方法简便、快捷,可用于开展临床研究。更多还原

【Abstract】 Objective:To develop HPLC method for the determination of terbutaline enantiomers in human plasma.Methods: The HPLC condition was column Waters Atlantis C18(4.6 mm×150 mm,5 μm),and water-acetonitrile(95∶5) contaning 8.4 mmol·L-1 β-CD(used as a chiral mobile phase additive),0.05 mmol·L-1ammonium acetate with pH 3.0 as the mobile phase with a flow rate at 0.6 mL·min-1.The chromatography was monitored by fluorescence detection(Ex=280 nm,Em=320 nm);column temperature was 10℃.Results: The linear range were 0.25～25 mg·L-1,the recovery was 99.35%～109.76%,inter-day RSD was less than 4% and intra-day RSD was less than 7%.Conclution: This method is simple and rapid.It provides a suitable method in clinical assay of terbutaline enantiomers.更多还原