【摘要】 目的:研究8-硝基白杨素(8-nitrochyrsin,NOChR)对HL-60细胞生长和凋亡的影响。方法:MTT法检测HL-60细胞增殖活性;台盼蓝染色细胞计数法测定细胞存活率,绘制细胞生长曲线;PI染色流式细胞术分析细胞周期和凋亡率。结果:NOChR呈浓度依赖性对HL-60细胞增殖具有抑制作用;NOChR显著抑制HL-60细胞生长;PI染色流式细胞术结果表明以浓度依赖方式诱导HL-60细胞凋亡,且使细胞周期阻滞于G1期。结论:NOChR具有诱导HL-60细胞凋亡的作用,其机制可能与细胞周期G1期阻滞相关。更多还原

【Abstract】 Objective:To investigate the effect of 8-nitrochyrsin(NOChR) on the growth and apoptosis of HL-60 cells cultured in vitro and its mechanism.Methods:8-nitrochyrsin on proliferation of HL-60 cells was detected by MTT assay.The trypan blue exclusion method was used to count the number of viable and dead cells,then the survival rate and growth curve were achieved.The apoptosis rate and cell cycle of HL-60 cells were determined by PI staining flow cytometry.Results:8-nitrochrysin inhibited proliferation of HL-60 cells in a concentration-dependent manner.The trypan blue exclusion method hint 8-nitrochyrsin posses the inhibitory effect of the growth of HL-60 cells.PI staining flow cytometry showed that cell cycle was arrested at G1 phase and the apoptosis of HL-60 cells was induced by NOChR in a concentration-dependent manner.Conclusions:NOChR posseses induction of apoptosis of HL-60 cells in vitro,and its mechanism may be associated with G1 phase cell cycle arrest.更多还原