【摘要】 目的:建立控制豨莶液质量的方法。方法:采用HPLC法定量测定豨莶液中的奇任醇含量。色谱条件:色谱柱为Allsphere ODS(4.6mm×250mm,5μm),流动相为乙腈-水-醋酸-三乙胺(25∶75∶0.1∶0.2),流速为1.0mL/min,检测波长为215nm,进样量为10μL,柱温为20℃。定性鉴别采用薄层色谱法。结果:在本色谱条件下,奇任醇在0.4168~1.6672μg范围内呈良好的线性关系。平均回收率为99.78%(RSD=1.51%,n=6)。薄层色谱中豨莶草的色谱斑点清晰、无干扰。结论:本法定性定量结合,准确可靠,重现性好,快速简便,可用于豨莶液的质量控制。更多还原

【Abstract】 Objective:To establish a method of controlling the quality for "Xixian Ye" that is a formulation of Traditional Chinese Medicine made of Herba Siegesbeckiae.Methods:HPLC was adopted for determining the Kirenol content of this formulation,in which Allsphere ODS C18 column(4.6 mm×250 mm,5 μm)and acetonitrile-water-acetic acid-triethylamine(25∶75∶0.1∶0.2)were used as the mobile phase at a flow rate of 1.0 mL/min,with a detection wavelength of 215 nm and a injection volume of 10μL and column temperature of 20 ℃.Moreover,TLC was adopted for qualitative identification of "Xixian Ye".Results:Under these chromatographic conditions,a good linearity relationship with the peak areas was exhibited(r= 0.999 5)while the sample size of Kirenol was in the range of 0.416 8～1.667 2 μg.The average recovery of Kirenol was 99.78 %(RSD=1.51 %,n=6).And a legible spot of Herba Siegesbeckiae was got by TLC.Conclusion:This method is accurate and reliable.It can effectively control the quality of the Chinese Traditional Medicine Formulation——"Xixian Ye".更多还原