【摘要】 目的:建立同时测定白花蛇舌草中2-甲基-3-羟基-4-甲氧基蒽醌（Ⅰ）和2-甲基-3-羟基蒽醌（Ⅱ）含量的高效液相色谱方法。方法:采用 Diamonsil C₁₈色谱柱（200 mm×4.6 mm,5μm）,以甲醇-水（62:38,v/v）为流动相,流速1.0 mL·min^-1,检测波长267 nm,柱温为室温。结果:Ⅰ和Ⅱ浓度分别在0.1～5.0μg·mL^-1（r=0.9999）和0.2～10.0μg·mL^-1（r=0.9999）范围内线性关系良好;加样回收率（n=6）分别为99.2%（RSD=1.9%）和99.8%（RSD=2.3%）。结论:本法准确可靠,重复性好,适用于白花蛇舌草中Ⅰ和Ⅱ的含量分析。更多还原

【Abstract】 Objective:To develop an HPLC method for quantitative determination of 2-methyl-3-hydroxy-4 -methoxyanthraquinone（Ⅰ）and 2-methyl-3-hydroxyanthraquinone（Ⅱ）in Hedyotis diffusa Willd.Methods: A Diamonsil C₁₈ column（200 mm×4.6 mm,5 μm）was used with a mobile phase of methanol-water（62:38,v/ v）at the flow rate of 1.0 mL·min^-1;The detection wavelength was set at 267 nm and the column temperature was room temperature.Results:The calibration curves were linear in the ranges of 0.1-5.0 μg·mL^-1（r=0.9999） for Ⅰ and 0.2-10.0 μg·mL^-1（r=0.9999）for Ⅱ.The average recoveries（n=6）of Ⅰ and Ⅱ were 99.2% and 99.8%,respectively.Conclusion:The method is accurate,reproducible for determination of Ⅰ and Ⅱ in Hedyotis diffusa Willd.更多还原