【摘要】 目的:建立 HPLC 法测定肿瘤细胞中酒石酸长春瑞滨的浓度。方法:采用氰基柱（4.6 mm×250 mm,5μm）,以乙腈-磷酸盐缓冲溶液(0.1 mol·L^-1磷酸二氢钾溶液,用磷酸调节 pH 至3.5)（25:75）为流动相,流速为1.0 mL·min^-1,检测波长为268 nm,柱温25℃。结果:线性范围为0.0315～10.1μg·mL^-1。（r=0.9996）;回收率（n=5）大于92.1%;日间、日内精密度试验的 RSD（n=5）均小于5.0%。结论:本试验建立的方法简便、快捷、专属性强,可用于临床研究酒石酸长春瑞滨的耐药机制,也可用于酒石酸长春瑞滨的药代动力学的研究。更多还原

【Abstract】 Objective:To establish an HPLC method to determine vinorelbine tartrate in tumor cells.Method:The column:Zorbax SB-CN column （4.6 mm×250 mm,5 μm）;the mobile phase:acetonitrile-phosphate buffer solu- tion （pH 3.5） （25:75）;the flow rate:1.0 mL·min^-1;the detection wavelength:268 nm;the column temperature: 25 ℃.Results:The calibration curves were linear between 0.0315-10.1 μg·mL^-1（r=0.9996）;the recovery （n =5） was more than 92.1%;intra-day RSD and inter-day RSD were both less than 5.0% （n=5）.Conclusion: The method is convenient and rapid with good specialization.It can be used for drug fastness mechanism study and pharmacokineties study of vinorelbine tartrate.更多还原