【摘要】 目的:采用胶束电动毛细管电泳法（MECC）分离腺嘌呤（A）、鸟嘌呤（G）、胞嘧啶（C）、胸腺嘧啶（T）、茶碱和咖啡因6种生物碱基。方法:20℃、18 kV 应用电压下,检测波长270 nm,在 35 mmol·L^-1十二烷基硫酸钠（SDS）-10 mmol·L^-1硼砂（pH 9.0）运行缓冲液中电泳分离。结果:6种碱基8 min 内达到基线分离;在10～500 mg·L^-1浓度范围内呈良好线性关系;相关系数为0.9995～0.9999;检出限为12.5～100μg·L^-1,加样回收率在96.1%～105.2%。并用于3种药物和茶叶中生物碱基含量的测定。结论:方法简便、快速,灵敏度高,可用于分析和测定含生物碱基的药物和饮料。更多还原

【Abstract】 Objective:To investigate the application of micellar electrokinetic capillary chromatography for separa- tion and determination six biological bases [cytosine （C）,thymine （T）,adenine （A）,caffeine,theophylline and gua- nine（G）].Method:At 20 ℃ and 18 kV applied voltage,on-line UV detector was operated at 270 nm,six biologi- cal bases introduced into the capillary separation reached the basesline separation in 8 minutes in 35 mmol·L^-1 SDS-10 mmol·L^-1 sodium tetraborate buffer solution （adjusted to pH 9.0 with hydrochloric acid）.Results:The concentration ranges of the linearity were all about 10-500 mg·L^-1,and the correlation coefficient was 0.9995- 0.9999,the limit of detection（S/N=3）was 12.5-100 μg·L^-1,the recovery was 96.1%-105.2%.This meth- od was adopted for the determination of biological bases in Qutong tablets,ganciclovir for injection,Xinganbao cap- sulcs and tea.Conclusion:The method is simple,rapid and accurate.更多还原