【摘要】 目的:用 HPLC 法同时测定双黄消炎片中黄芩苷和3种生物碱类成分药根碱、巴马汀及小檗碱的含量。方法:色谱柱:Diamonsil^TM（钻石）C₁₈柱（250 mm×4.6 mm,5μm）;流动相:0.1%磷酸（加三乙胺调 pH 至2.97）-乙腈（75:25）;流速:1.0 mL·min^-1;检测波长:345 nm。结果:黄芩苷、药根碱、巴马汀和小檗碱分别在0.13～2.7μg（r=0.9998）,0.0097～0.19μg（r=0.9998）,0.010～0.20μg（r=0.9999）,0.020～0.40μg（r=0.9998）范围内线性关系良好;4个成分的平均回收率（n=9）分别为100.4%,99.7%,100.1%,100.4%。结论:本方法快速、简便,重复性好,可作为该制剂的质量控制方法。更多还原

【Abstract】 Objective:To determine the contents of baicalin,jatrorrhizine,palmatine and berberine in Shuanghuang Xiaoyan tablets by HPLC.Methods:Using an HPLC method with Diamonsi]^TM C₁₈ column （250 mm ×4.6 mm,5 μm） ,0.1% phosphoric acid （triethylamine adjusted to pH 2.97）-acetonitrile （75:25） as mobile phase,flow rate was 1.0 mL·min^-1,with the detection wavelength at 345 nm.Results:The calibration curves of baicalin,ja- trorrhizine,palmatine and berberine were linear within the range of 0.13-2.7 μg（r=0.9998）,0.0097-0.19 μg （r=0.9998）,0.010-0.20 μg （r=0.9999）,0.020 -0.40 μg（r=0.9998）respectively.The average recoveries （n=9） were 100.4%,99.7,100.1%,100.4%.Conclusion:The method is rapid,sensitive and better reproduc- ibility.It can be used to control the quality of Shuanghuang Xiaoyan tablets.更多还原