【摘要】 目的:建立测定当归中阿魏酸含量的 HPLC 法。方法:采用 Symmetry C₁₈柱（4.6 mm×150 mm,5μm）,流动相为乙腈-0.02 mol·L^-1磷酸三氢钾溶液（17:83,以磷酸调节 pH=2.50）,流速1.0 mL·min^-1,检测波长322 nm,温度:35℃。结果:阿魏酸进样量在0.05～0.13μg范围内具有良好的线性关系（r=0.9998）;半均回收率（n=5）为101.1%,RSD 为3.3%。结论:该方法简便、准确,灵敏度高,重复性好。更多还原

【Abstract】 Objective:To establish an RP-HPLC for quantitative determination of ferulic acid in Radix Angelicae Sinensis.Methods:Ferulic acid was performed on Symmetry C₁₈ column（4.6 mm×150 mm,5μm）with mobile phase of acetonitrile-0.02 mol·L^-1 potassium dihydrogen phosphate buffer solution（17:83,adjusted to pH= 2.50 with phosphoric acid）at a flow rate of 1.0 mL·min^-1,detected at 322 nm and column temperature 35℃. Results:The good linear range of ferulic acid was 0.05-0.13μg,r=0.9998.The average recovery（n=5）was 101.1%,and relative standard deviation was 3.3%.Conclusion:This method is testified to be simple,accurate, sensitive and the reproducibility is good.更多还原