【摘要】 目的:建立红豆杉中紫杉醇的提取纯化方法和紫杉醇高效液相色谱分析方法,分析研究了我国境内中国红豆杉、云南红豆杉、西藏红豆杉、东北红豆杉、南方红豆杉以及曼地亚红豆杉不同部位紫杉醇含量的差异,寻找其分布规律。方法:以乙酸乙酯-丙酮（1∶1）为提取溶剂进行提取,采用自制碱性氧化铝柱进行柱层析纯化;色谱条件:采用 AichromBond-AQ C₁₈（250 mm×4.6 mm,5μm）分析柱,以甲醇-乙腈-水（18∶40∶42）为流动相,流速1.0 min·mL^-1,柱温40℃,于228 nm 波长处进行检测。结果:红豆杉各部化紫杉醇含量差异明显,其中叶、枝条、根和树皮的平均含量分别为0.015%,0.018%,0.055%,0.077%。中国红豆杉、云南红豆杉、西藏红豆杉、东北红豆杉、南方红豆杉以及曼地亚红豆杉树木各部位的平均含量分别为0.032%,0.050%,0.034%,0.043%,0.044%,0.046% 结论:鉴于红豆杉的枝和叶中也含有相当量的紫杉醇且再生能力较强,故应以红豆杉的枝叶为原料进行提取、分离,对红豆杉的保护和合理开发利用都具有现实意义,其中尤以云南红豆杉开发利用价值最高。更多还原

【Abstract】 Objective:To estahlish an HPLC method for the extraction and determination of taxol from Taxus grow- ing in China.The taxol centent in different parts of six species was analysed and compared.Method:The samples u- sing ethyl acetate-acetone（1:1）as the extract solvent.The analysis was performed on AichromBond-AQ C₁₈ col- umn（250 mm×4.6 mm,5μm）with mobile phase containing methanol-acetonitrile-water（18:40:42）.The flow rate was 1.0 mL·min^-1.The column temperatnre was 40℃.The detected wavelength was set at 228 nm.Result: The average taxol contents of leaves,branches,roots and barks were 0.015%,0.018%,0.055% and 0.077% re- spectively.And the average taxol contents of this six species were 0.032%,0.050%,0.034%,0.043%,0.044% and 0.046% respectively.Conclusion:In view of the branches and leaves regeneration ability is so strong that it shouht be use as raw materials extraction,separation in production.The protection and rational use of Taxus have practical significance.In particular,the development of Taxus yuannanensis is the most valuable.更多还原