【摘要】 目的:建立复方金刚痛风颗粒中菝葜的TLC鉴别方法和含量测定方法。方法:以薯蓣皂苷元为对照品,采用氯仿-乙酸乙酯(10:1)为展开剂,10%硫酸乙醇溶液为显色剂,对复方金刚痛风颗粒中的菝葜进行了TLC鉴别;采用大连依利特C18色谱柱(Hypersil ODS,5μm,4.6×250mm),乙腈-水(90:10)为流动相,流速为1mL.min-1,203nm为检测波长,柱温为35℃,测定了复方金刚痛风颗粒中薯蓣皂苷元的含量。结果:薯蓣皂苷元斑点清晰,阴性对照无干扰。薯蓣皂苷元线性范围为0.212μg～1.908μg(r=0.9999),平均回收率为100.50%,RSD为1.49%。方法灵敏度高、结果准确、重现性好。结论:所建立的方法简便可行、重现性好,为复方金刚痛风颗粒质量控制提供了有效途径。更多还原

【Abstract】 Objective:To establish TLC identification method and HPLC assay method for Smilax China L of compound gout granules.Methods:Smilax china L were identified by TLC respectively,using diosgenin as reference substances,and(10∶1) as mobile phases.Diosgenin were determined by HPLC using the Hpersil ODS reversed-phase column of C18(4.6×250mm,5μm),the mobile phase was a combination of acetonitrile and water(90∶10) at the flow rate of 1.0mL·min-1,and the eluate was monitored with UV absorption at 203nm,retention at 35 degrees.Result:The TLC chromatograms of diosgenin were of clearness,good separation,and no interference.The diosgenin contents in Compound gout granules by HPLC,in the linear range of 0.212μg to 1.908μg(r=0.9999) and the recoveries of 100.50%(RSD=1.49%).Conclusion:The established methods are simple,feasible and reproducible.This study provide a method for the quality control of compound gout granules更多还原