【摘要】 目的:建立测定银杏缓释片中总黄酮苷含量的方法。方法:采用高效液相色谱法,用DiamonsilTM-0DS柱,以甲醇-0.5%磷酸(52:48)为流动相,检测波长360nm,流速1ml/min,保持柱温40℃测定自制银杏缓释片中总黄酮苷的含量。结果:槲皮素在5～60μg/mL内线性关系良好,回收率为99.7%,RSD为0.93%。结论:本法测定银杏缓释片中总黄酮苷的含量,结果准确、可行。更多还原

【Abstract】 Objective:To establish assaying methods for the determination of total flavonoid glycoside in Ginkgo biloba sustained-release tablets.Method:External standard method was conducted with the column of C18(250×4.6 mm,5?m),mobile phase of methanol-0.5% orthophosphoric acid(52:48),flow rate of 1.0ml/min,and detection wavelength was at wavelength of 360nm.Results:In the range of 5～60μg,the distribution of quercetin is linear(r=0.9998),with an average recovery of 99.7%±1.3%(RSD=0.93%).Conclusion:The assaying method of RP-HPLC is convenient,accurate,and reliable with good precision,and can be used in the quality control of Ginkgo biloba sustained-release tablets.更多还原