【摘要】 以文山红柱兰种子为外植体,进行了种子无菌萌发,球茎丛生芽继代增殖、无根苗生根培养等的组培技术研究。其研究结果表明:在1/2 MS+0.2 mg/LBA+0.2 mg/LNAA的培养基上文山红柱兰种子的萌发效果最好;在1/2 MS+BA1.5 mg/L+NAA0.2 mg/L+80 g/L香蕉泥+1 g/L花宝4号培养基上进行增殖培养效果较好,在1/2 MS+0.6 mg/LNAA+0.2 mg/LBA+0.3%活性炭培养基的生根培养效果较好。苗高6~8 cm时可出瓶炼苗。更多还原

【Abstract】 Using seeds as explants,tissue culture technique of C.wenshanense including sterile seed germination,regeneration and rooting was experimented and studied.The experiment results showed that the sterile seed germination medium was 1/2 MS+0.2 mg/L BA+0.2 mg/L NAA,the medium for regeneration was 1/2 MS + 1.5 mg/L BA+0.2 mg/L NAA+80 g/L mashed banana+1 g/L Huabao No.4,the medium for root inducing was 1/2 MS+0.6 mg/L NAA+0.2 mg/L BA+0.3 % AC.The tissue culture plants of 6 to 8 cm high could be transplanted.更多还原