【摘要】 对粉拟青霉菌菌株的毒力复壮技术和其复壮菌的应用效应进行了系统的研究。经不断的探索获得了粉拟青霉菌复壮用的Pf-Ⅱ号培养基,对其复壮菌株作室内及林间的毒力测试,结果显示:(1)经复壮后的粉拟青霉菌菌株对松纵坑切梢小蠹成虫的感病死亡率为88.5%,比未经复壮的菌株感病死亡率48.8%提高了39.7%。(2)林间温度对粉拟青霉菌使松纵坑切梢小蠹成虫感病死亡的影响不明显,但林间使用该菌防治松纵坑切梢小蠹成虫的适宜温度为15℃~20℃;粉拟青霉菌致使松纵坑切梢小蠹成虫感病的死亡率与林间相对湿度成正相关,最适相对湿度为90%~95%,下限不能低于80%;1月中旬、6月下旬至10月下旬是应用粉拟青霉菌防治松纵坑切梢小蠹的最佳时期。用复壮后的粉拟青霉菌菌粉在云南省沾益县西平镇文家坟、火石梁子的150 hm2蠹害林区对云南松纵坑切梢小蠹进行防治。其林分的松纵坑切梢小蠹成虫受害株率由防治前的32.9%降至4.6%,受害株减退率为86.1%,进一步表明:用毒力复壮后的粉拟青霉菌,防治松纵坑切梢小蠹虫虫害的效果显著。更多还原

【Abstract】 The method for virulence rejuvenescence of Paecilomyces farinosus and application techniques of rejuvenescence Paecilomyces farinosus in forest were studied.Using the rejuvenescence Paecilomyces farinosus to control the Tomicus piniperda in Pinus yunnanensis stand of 150 ha located at Wenjiafen and Huoshiliangzi of Zhanyi county of Yunnan province,the following results were achieved.The fatality rate of rejuvenescence Paecilomyces farinosus on Tomicus piniperda was 88.5 %,and that of Paecilomyces farinosus without rejuvenescence treatment was only 39.7 %.The effect of temperature on control effect of Paecilomyces farinosus on Tomicus piniperda was not significant,and the optimum temperature scope was 15 ~ 20℃,the optimum relative humidity was 90 % ~ 95 %,with the lower limit of 80 %.Mid January,last third of June and last third of October were the optimum time for Tomicus piniperda control using Paecilomyces farinosus.The effect of controlling Tomicus piniperda by using Paecilomyces farinosus at the experimental sites afforementioned,the percentage of Tomicus piniperda damaged individuals decreased from 32.9 % to 4.6 %,which implied the significant control effect in forest.更多还原