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山羊mtDNA多态性及其遗传结构的研究

Research on the Genetic Diversity and Structure of mtDNA of 6 Indigenous Goat Breeds in China

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【作者】 季香马月辉叶绍辉郭军

【Author】 JI Xiang1,2,MA Yue-hui1,YE Shao-hui2,GUO Jun1(1.Institution of Animal Science,China Academy of Agricultural Science,Beijing 100094,China;2.Faculty of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China)

【机构】 中国农业科学院畜牧研究所云南农业大学动物科学技术学院中国农业科学院畜牧研究所 北京100094云南昆明650201北京100094

【摘要】 对中国6个山羊品种(辽宁绒山羊,内蒙古绒山羊,西藏山羊,中卫山羊,济宁青山羊和龙陵山羊)的52个样本进行m tDNA的D-Loop区的全序列测定,用来研究中国山羊品种的线粒体DNA遗传多态性及系统发育。用NJ法构建单倍型系统发育树,结果发现6个山羊品种的线粒体DNA控制区聚类后得到了4个很明显的分支,即单倍型A,B,C,D.品种间遗传结构分析发现内蒙古绒山羊、辽宁绒山羊两个绒山羊品种与西藏山羊、中卫山羊3个可产绒的山羊品种聚在一起,接着与产羔皮的济宁青山羊聚在一起,最后与产肉性能强的龙陵山羊聚在一起。

【Abstract】 In this paper,52 individuals were chosen from 6 indigenous goat breeds raised in China.Fifty-two individuals’ complete sequences of D-Loop,the control region of mitochondrial DNA(mtDNA),were sequenced in order to study mtDNA genetic diversity and phylogenetic evolution of these 6 breeds.The phylogenetic tree of D-Loop was constructed with Neighbour-Joining(NJ) method and the genetic structure among the 6 breeds was studied by cluster analysis based on the genetic distance.The results showed that all of the domestic haplotypes were divided into 4 high obvious divergent clusters(A,B,C and D) and the cluster analysis showed a closer relationship among LNC,NMC,TiG and ZW goats and a farther relationship between LL goat and other goat.

【关键词】 山羊mtDNA遗传多样性系统发育
【Key words】 goatmitochondrial DNAgenetic diversityphylogeny evolution
【基金】 国家自然科学基金项目(30371026);国家自然科技资源平台项目(2005DKA21101)
  • 【文献出处】 云南农业大学学报 ,Journal of Yunnan Agricultural University , 编辑部邮箱 ,2008年02期
  • 【分类号】S827
  • 【被引频次】5
  • 【下载频次】146
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