【摘要】 通过研究退火温度与引物TM值之间的关系,确定玉米核心引物设计时对TM值的特殊要求,通过比较3种PCR扩增程序,确定两步法扩增作为新设计引物的统一的PCR反应程序,为基于玉米SSR核心引物构建多重PCR组合反应进行高通量的基因分型奠定了基础。更多还原

【Abstract】 The special request to TM value for maize SSR core primer was determined according to relationship between annealing temperature and primer’s TM value,and two-step PCR program was determined as a common program for new-designed primers after three PCR programs compared,which help to multiplex PCR and high-throughput genotyping with maize SSR core primers.更多还原