【摘要】 目的:研究异丙酚不同麻醉深度对兔脑神经元型一氧化氮合酶(nNOS)mRNA水平的影响。方法:30只日本大耳兔,随机分为3组:对照组,浅麻醉组,深麻醉组,每组10只。浅麻醉组、深麻醉组予以异丙酚靶控输注,达到所需麻醉状态后1h断头处死。对照组行耳缘静脉注射空气造成空气栓塞后致死。动物处死后0~4℃分离取脑,原位杂交法测定小脑皮质、大脑顶叶皮质nNOS mRNA表达水平。结果:与对照组比较,异丙酚麻醉状态下明显抑制兔大脑顶叶皮质、小脑皮质nNOS mRNA表达水平(P<0.05)。随麻醉深度的加深,小脑皮质nNOS表达水平进一步明显降低(P<0.05),但大脑顶叶皮质nNOS表达水平并未进一步降低(P>0.05)。结论:异丙酚可能通过抑制nNOS活性,降低NO、环磷酸鸟苷(cGMP)水平,从而发挥麻醉作用。异丙酚对nNOS活性的抑制存在脑区差异。更多还原

【Abstract】 AIM: To investigate alterations of nNOS mRNA in discrete brain regions of rabbits under propofol anesthesia.METHODS: Thirty rabbits were randomly allocated to three groups with ten each: control group,light anesthetic group,deep anesthetic group.Experimentation groups achieved needing drugged state by using target-controlled infusion of propofol,after one hour the rabbits were executed.Control group ear intravenous injection air caused aeroembolism death.Three groups rabbits were isolated to obtain brains under 0-4 ℃,the levels of nNOS mRNA in cerebellar cortex,parietal cortex were observed under different anesthetic depths.RESULTS: Comparing with control group,the levels of nNOS mRNA both in light anesthetic group and in deep anesthetic group decreased significantly in cerebellar cortex and parietal cortex(P<0.05) of rabbit brain.With the increasing of anesthetic depth,significant decreases in nNOS mRNA levels were observed in cerebellar cortex(P<0.05),but no significant difference was found in parietal cortex(P>0.05).CONCLUSION: Propofol probably exerts its anesthetic action through depressing the activity of nNOS,decreasing the levels of NO and cGMP.The depression differs in different brain regions.更多还原