【摘要】 目的为了探讨血清胰岛素(INS)和C肽(C-P)释放试验放射免疫分析法(RIA)与时间分辨免疫荧光分析法(TRIFA)检测结果的差异及各自优势。方法采用RIA法和TRFIA法对30例糖尿病患者的空腹﹑餐后30分钟﹑60分钟﹑120分钟血清INS和血清C-P水平进行检测。结果糖尿病组血清胰岛素空腹﹑餐后30分钟﹑60分钟﹑120分钟TRFIA法测定值显著低于RIA法测定值(p〈0.01);血清C肽空腹﹑餐后30分钟﹑60分钟﹑120分钟TRFIA法测定值显著高于RIA法测定值(p〈0.01)。结论RIA法与TRFIA法检测结果具有显著性差异,表明两种方法的INS﹑C-P的测定结果不能混用。更多还原

【Abstract】 Objective To determine the difference and advantage of Serum Insulin(INS)and C-P by radioimmunoassay(RIA)and Time-resolved Fluorescence Immunoassay(TRFIA). Methods The concentrations of serum INS and C-P at fasting、postprandial 30`m、postprandial 60`m and postprandial 120`m was detected by RIA and TRFIA in 30 diabetic patients. Results The determinations of serum INS and C-P at fasting、postprandial 30`m、postprandial 60`m and postprandial 120`m by TRFIA was lower than the one by RIA(P<0.01). Conclusion The determinations was significant differences by RIA and TRFIA, that the results of the two methods can not mix.更多还原