【摘要】 目的比较番茄红素及维生素(Vit)E和VitC合用对庆大霉素大鼠肾毒性的预防作用。方法雄性SD大鼠给予庆大霉素140 mg.kg-1(ip),连续7 d。预防组同时予以番茄红素20 mg.kg-1(ig)或Vit E 50 mg.kg-1和Vit C 200 mg.kg-1(ig)。给药7 d后,测定大鼠血清尿素氮(BUN)、血肌酐(SCr)和尿肌酐含量,计算肌酐清除率(CCr);测定尿N-乙酰-β-D-氨基葡萄糖苷酶(NAG)活性,肾组织中超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性及丙二醛(MDA)含量;HE染色观察肾脏病理改变。结果与正常对照组比较,庆大霉素组大鼠血清BUN和SCr含量升高,CCr降低,尿NAG活性升高,肾组织MDA水平增加,SOD和CAT活性降低,肾脏病理改变明显。给予番茄红素,或Vit E和Vit C合用明显减轻上述改变,番茄红素的作用较Vit E和Vit C合用更为明显。结论番茄红素及Vit E和Vit C合用均可能通过抗氧化应激作用减轻庆大霉素大鼠肾毒性,番茄红素的作用可能优于Vit E和Vit C合用。更多还原

【Abstract】 AIM To investigate the possible protective effects of lycopene and combination of vitamin(Vit) E and Vit C against gentamicin-induced nephrotoxicity in rats.METHODSMale SD rats were given gentamicin 140 mg·kg-1(ip),gentamicin+lycopene 20 mg·kg-1(ig) and gentamicin+Vit E 50 mg·kg-1+Vit C 200 mg·kg-1(ig),respectively,once daily for 7 d.Serum creatinine(SCr),blood urea nitrogen(BUN),creatinine clearance rate(CCr) and N-acetyl-beta-D-glucosamidase(NAG) in urine were assessed,as well as the malondialdehyde(MDA) level,superoxide dismutase(SOD) and catalase(CAT) activities in renal tissue were determined.The renal morphology changes were observed by hematoxylin-eosin staining.RESULTS Gentamicin induced a significant increment in SCr,BUN,NAG and MDA contents and severe morphology changes,decreased the renal SOD and CAT activities.Lycopene and combination of Vit E and Vit C significantly reversed the changes mentioned above.And lycopene was superior to combination of Vit E and Vit C.CONCLUSION Lycopene and combination of Vit E and Vit C provide marked protective effect against gentamicin-induced nephrotoxicity in rats,which may be related to their antagonistic effects on oxidative stress.Lycopene is more powerful than combination of Vit E and Vit C.更多还原