【摘要】 目的小补心汤(XBXT)由代赭石、旋覆花、竹叶和淡豆豉4味中药组成,文献记载其有缓解抑郁情绪的作用。本实验研究其总黄酮提取物(XBXT-2)是否具有抗抑郁作用。方法采用大、小鼠强迫游泳模型和大鼠获得性无助模型观察XBXT-2的抗抑郁作用,采用酶联免疫法检测获得性无助大鼠血清皮质酮水平,并观察XBXT-2对小鼠自发活动的影响。结果单次灌胃给予XBXT-2 50和100mg.kg-1可以显著缩短小鼠和大鼠强迫游泳的不动时间。连续4 d灌胃给予XBXT-2 25和50 mg.kg-1可以显著减少获得性无助模型大鼠的逃避失败次数,并显著降低其血清皮质酮水平。XBXT-2 50 ～200 mg.kg-1对正常小鼠的自发活动性无明显影响。结论 XBXT-2在抑郁动物模型上具有抗抑郁样作用,其机制可能与其抑制下丘脑-垂体-肾上腺轴功能亢进有关。更多还原

【Abstract】 AIM Xiaobuxin-Tang(XBXT) is a traditional Chinese herbal decoction which is composed of Haematitum,Flos Inulae,Folium Phyllostachydis Henonis and Semen Sojae Preparatum.The present study was to investigate if the total flavonoids extracted from XBXT(XBXT-2) had antidepressant effect.METHODS Forced swimming tests in mice and rats,and learned helplessness(LH) model of rats were adopted to affirm the antidepressant effect of XBXT-2 with the test on spontaneous motor activity.Plasma corticosterone level in the LH rats was measured with ELISA.RESULTS Single administraton of XBXT-2 at the doses of 50 and 100 mg·kg-1(ig) significantly decreased the duration of immobility time in the forced swimming tests in mice and rats.Researches on LH model of rats indicated that XBXT-2 at doses of 50 and 25 mg·kg-1 markedly reduced the number of escape failure in shuttle box.Meanwhile,the plasma corticosterone level of the LH rats was significantly decreased.XBXT-2 50-200 mg·kg-1 had no effects on spontaneous motor activity in mice.CONCLUSION XBXT-2 possesses significant antidepressant-like effect.The mechanism may involve the inhibition of the hyperaction of the hypothalamic-pituitary-adrenal axis.更多还原