【摘要】 目的研究抗肝硬化复方(BJ-JN)在体外对活化星状细胞(HSC)增殖和胶原合成的抑制作用,进一步探讨BJ-JN的作用机制。方法在引入血清药理方法的基础上,探讨BJ-JN在体外对混合新生小牛血清(NBS)+大鼠血清(RS)和转化生长因子β1(TGF-β1)两种刺激因素作用下的HSC-T6细胞增殖和胶原合成的抑制作用。将不同时间点(2.5、3.5 h)采集的BJ-JN含药血清作用于NBS和RS共同刺激下的HSC-T6,分别加入3H-TdR和3H-Pro,在液体闪烁计数仪上检测HST-T6细胞增殖和胶原合成。在5个不同的时间点(2~24 h)将刺激浓度为9 pmol/L的TGF-β1作用于HSC-T6,用四甲基偶氮唑(MTT)比色法测定吸光度值,确定TGF-β1刺激HST-T6的最适刺激时间。将不同浓度(0.19%~3.125%)BJ-JN含药血清作用于最适刺激时间、9 pmol/LTGF-β1刺激条件下的HST-T6,用MTT比色法测定吸光度值并计算抑制率。结果与正常对照组比较,给药2.5和3.5h BJ-JN含药血清对NBS+RS刺激的HSC-T6细胞增殖和胶原合成有显著的抑制作用。9 pmol/L TGF-β1对HSC-T6细胞作用16 h的刺激作用最强。不同浓度BJ-JN含药血清可显著抑制TGF-β1刺激的HSC-T6细胞增殖。结论BJ-JN含药血清在体外可以明显抑制HSC-T6细胞增殖和胶原合成。更多还原

【Abstract】 Objective To study the inhibitory effect of BJ-JN on proliferation and collagen synthesis of active HSC cells in vrtro and its mechanism.Methods The inhibitory effect of BJ-JN on the proliferation and collagen synthesis of HSC-T6 cells was investigated,which was stimulated by mixed sera and transforming growth factor β1 respectively,by serum pharmacology method.The effect of serum containing BJ-JN in different time(2.5,3.5 h) on the proliferation and collagen synthesis of HSC-T6 cells costimulated by mixed sera containing new bovine serum(NBS) and rat serum(RS) was measured by -TdR and -Proline incorporation assay.In additionally,the effect of TGF-β1(9 pmol/L) in different stimulus durations(2~24 h)and the effect of drug-contained serum of BJ-JN in different concentrations(0.19%~3.125%) on proliferation of HSC-T6 stimulated by TGF-β1(9 pmol/L) in the optimal stimulus duration was measured by MTT assay.Results Drug-contained serum of BJ-JN in 2.5 h and 3.5 h could markedly suppress proliferation and collagen synthesis of HSC-T6 cells stimulated by mixed sera.The optimal stimulus duration of TGF-β1(9 pmol/L) on HSC-T6 was 16 h.Drug-contained serum of BJ-JN could markedly inhibit the activation and proliferation of HSC-T6 cells induced by TGF-β1(9 pmol/L).Conclusion These results suggest that BJ-JN has inhibitory effect on proliferation and collagen synthesis of active HSC-T6 cells in vitro.更多还原