【摘要】 Activator protein-1(AP-1)是重要的转录因子,其活性失调与肿瘤等多种疾病直接相关。本文运用"高通量高内涵细胞筛选技术(high throughput-high content cell-based screening technology)"对650个以未知功能基因为主的人类基因进行AP-1双荧光素酶报告基因筛选(Dual-Luciferase reporter gene screening),获得了一个可抑制佛波酯(PMA)加离子霉素(Inonmycin)诱导的AP-1活性的人类新基因AC3-33(GenBank中该基因名为C3orf33,No.FLJ31139)。生物信息学分析该基因序列全长1931bp,由6个外显子和5个内含子组成,定位于3q25.31,从271～1026有一个编码251个氨基酸的可读框,编码一个约29kDa的蛋白,在肾上腺和宫颈等多种组织都有表达。AC3-33与其他人类已知蛋白质没有明显的同源性,亚细胞定位于细胞质中,许多氨基酸序列高度保守。初步实验结果显示AC3-33是一个有重要功能的人类新基因。更多还原

【Abstract】 Activator protein-1(AP-1)is an important transcription factor,and dysregulation of its activity has been associ-ated with many human diseases,including various cancers.A novel human gene AC3-33(GenBank name:c30rf33,Acces-sion No.FLJ31139),which can suppress PMA and Ionomycin induced activation of AP-1,was identified from 650 human function-known genes by using the high throughput-high content cell-based screening technology.The gene whose full cDNA length is 1391 bp containing 6 exons and 5 introns is located in the human chromosome 3q25.31.The predicted pro-tein encoded by this gene contains 251 amino acids with a theoretical molecular weight of 29 kDa.Expression of the AC3-33 gene is widly found in adrenal glands and cervix.The amino acid sequences of AC3-33 is highly conserved,and has no homology to other known proteins.Subcellular localization studies show that the AC3-33 protein was localized inthe cytoplasm.Our preliminary results showed that AC3-33 is an important novel gene related to supress AP-1 activity.更多还原