【摘要】 为调查HIV-1感染相关等位基因CCR2-64Ⅰ在我国南方14个少数民族群体的频率和多态性分布,从上述人群外周血中抽提基因组DNA,采用PCR和PCR-RFLP等方法进行基因分型。在791例调查对象中,636例是野生纯合子基因型,104例为杂合子基因型,51例为突变纯合子基因型。上述各群体等位基因型的分布符合Hardy-Weinberg平衡。14个民族群体的平均突变基因频率为13.6%,等位基因频率范围分布在1.6%~30.3%之间,14个民族群体之间突变基因频率具有显著差异(P<0.05)。广西壮族群体CCR2-64Ⅰ突变基因频率最低,为1.6%,云南的六库傈僳族频率最高,为30.3%。12个群体的突变基因频率均低于中国汉族健康群体,南方3个少数民族群体基因突变频率显著低于西南11个少数民族群体,该突变基因在艾滋病发病过程中的影响值得进一步深入研究。更多还原

【Abstract】 The purpose of the work is to investigate the frequencies and polymorphisms of HIV-1 resistant CCR2-64Ⅰ alleles in 14 Chinese populations in South China. The blood samples include 791 unrelated healthy individuals. Whole blood samples were collected randomly and their genomic DNA were extracted with OMEGA Blood Kits. Allelic frequen-cies were identified by PCR-RFLP analysis. 636 individuals were wild-type homozygous, 104 individuals were CCR2-64Ⅰ heterozygote and 51 individuals were CCR2-64Ⅰ homozygous. Distributions of the CCR2-64Ⅰalleles among the popula-tions were in accordance with Hardy-Weinberg equilibrium. The average allele frequencies was 13.6%, and the frequency span of the alleles was 1.6%~30.3%. By statistical analysis, the frequency varied significantly in 14 populations (P<0.05). Among them, Zhuang ethnic population had the lowest CCR2-64Ⅰallele, whereas the Lisu ethnic population in Yunnan province had the highest CCR2-64Ⅰallele Conclusion: The frequency of CCR2-64Ⅰallele of 12 ethnic populations were lower than that of Han. The frequency of CCR2-64Ⅰallele between Southern and South-east group showed significant variations. The mutant alleles conferring genetic resistance to HIV-1 and AIDS progression needs to be further studied.更多还原