【摘要】 目的研究全反式维甲酸(ATRA)与苯那普利对肾硬化大鼠肾脏组织α-SMA表达的影响。方法80只Wistar♂大鼠随机分为假手术组、模型组、苯那普利组和ATRA组,每组均为20只。采用单侧肾切除加1wk后尾静脉注射阿霉素(5mg·kg-1)的方案建立大鼠肾小球硬化(GS)模型,造模后12wk末处死。HE染色观察肾脏病理改变,计算肾小球硬化指数(GSI)。RT-PCR结合免疫组织化学方法检测肾组织α-平滑肌肌动蛋白(α-SMA)的表达情况。结果与模型组相比,ATRA组和苯那普利组GSI、α-SMA mRNA相对表达量和α-SMA阳性面积比均明显下降(P<0.05),但两组间各项指标比较无差异(P>0.05)。结论ATRA与苯那普利对大鼠肾硬化均有明显的延缓效应,且疗效一致。更多还原

【Abstract】 Aim To investigate the effects of all-trans retinoic acid and benazepril on the expression of α-smooth muscle actin in rats with glomerulosclerosis.Methods 80 Wistar male Rats were randomly assigned into the following groups: control group,model group,ATRA treatment group and benazepril treatment group,20 rats in each group.GS rats were uninephrectomized and injected with adriamycin(5mg·kg-1) after one week through the tail vein.All rats were sacrificed at the 12th week,GS was evaluated by glomerulosclerosis index(GSI) system.The expression of α-SMA was assessed by reverse transcription-polymerase chain reaction(RT-PCR) and immunohistochemistry.Results Comparing with control group,the expression of α-SMA mRNA and protein were decreased significantly in ATRA treatment group and benazepril treatment group(P<0.05),but obvious differences of every index were not observed between the two groups(P>0.05).Conclusions The postponed effects of ATRA and benazepril on GS were evident and equivalent.更多还原