【摘要】 目的apelin是新发现的内源性肽,其受体是G蛋白偶联受体APJ,该实验比较不同年龄大鼠心肌APJ受体及信号分子caveolin-1/ERK1/2的表达差异。方法采用West-ern blot方法观察胎鼠、乳鼠、成年鼠、老龄鼠等不同年龄阶段心肌组织APJ受体、ERK1/2、pERK1/2、caveolin-1的表达及其增龄性变化。结果随年龄增长APJ受体、caveolin-1表达增加,ERK1/2及pERK1/2随年龄增长表达减少。结论APJ受体及其caveolin-1/ERK1/2途径可能与心脏的生长发育、衰老过程有关。更多还原

【Abstract】 Aim To investigate the regulatory route and the role of APJ receptor in the growth and aging process of rat myocardium.Methods Western blot was used to observe the changes of APJ receptor,ERK, pERK and caveolin-1 expression.Results The findings showed that APJ receptor and caveolin-1 expression increased with age growth, but decreased slightly at the gerontism.The ERK and pERK expression decreased with age growth.Conclusion Apelin-APJ receptor-caveolin-1/ERK1/2 route may be involved in the growth and aging process of rat heart.更多还原