【摘要】 目的观察化学修饰的四环素(米诺环素、多西环素)对全氟异丁烯(perfluoroisobutylene,PFIB)吸入性急性肺损伤的防治效果。方法采用小鼠动态吸入PFIB周身暴露染毒装置对实验小鼠进行染毒,观察了化学修饰的四环素(chemical modified tetracyclines,CMTs)给药组与中毒对照组小鼠的肺系数以及支气管肺灌洗液(bronchoalveo larlavage fluid,BALF)中蛋白质含量的变化情况。结果米诺环素预防可以显著性地降低各项肺系数以及减少BALF中蛋白质的含量;多西环素预防可以显著性地减少BALF中蛋白质的含量,多西环素治疗可以显著性地降低湿肺体比和干肺体比。结论CMTs药物米诺环素对PFIB吸入性急性肺损伤具有预防作用,而多西环素对PFIB吸入性急性肺损伤具有较弱的预防和治疗作用。更多还原

【Abstract】 Objective To investigate the effects of chemical modified tetracyclines(minocycline and doxycycline)on acute lung injury(ALI)induced by perfluoroisobutylene(PFIB)inhalation in mice.Methods ICR mice were exposed to gaseous PFIB in a flow-past whole-body exposure system.Lung toxicity and mortality induced by PFIB inhalation were studied.Results Minocycline pretreatment(45 mg/kg,20 min before PFIB exposure)significantly reduced the lung index(wet/dry lung-to-body weight ratio,the water content in the lung and lung wet-to-dry weight ratio)and protein contents of bronchoalveolar lavage fluid(BALF)in ALI mice,while no effects were found when minocycline(45 mg/kg)was administrated 1 h after PFIB inhalation.When doxycycline was valuated,it was observed that doxycycline(50 mg/kg)administrated 1 h after PFIB exposure could significantly lower the wet/dry lung-to-body weight ratio,and the same dose of doxycycline given 20 min before PFIB exposure could significantly reduce the protein content of BALF.Conclusion A preventive effects of minocycline against the toxicity of PFIB inhalation was observed.A slight preventive and therapeutic effects of doxycycline against the toxicity of PFIB inhalation was observed.更多还原