【摘要】 目的:检测慢性乙型肝炎患者血清转化生长因子-β(TGF-β)、基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶组织抑制物-1(TIMP-1)水平及变化和肝纤维化程度及其变化,探讨其血清TGF-β、MMP-1、TIMP-1与肝纤维化及其进展的关系。方法:慢性乙型肝炎患者80例,行肝穿刺活检,病理组织进行纤维化程度半定量计分,6个月后复查;检测血清TGF-β、MMP-1、TIMP-1、IV型胶原(ⅣC)、层黏连蛋白(LN)、Ⅲ型前胶原N端肽(PⅢP)、透明质酸酶(HA)6个月后复查。结果:肝纤维化分级不同,血清TGF-β、TIMP-1、TIMP-1/MMP-1差异显著(P<0.01),MMP-1差异不明显(P>0.05);TGF-β(r=0.745,P<0.01)、TIMP-l(r=0.623,P<0.01)、TIMP-1/MMP-l(r=0.336,P<0.01)纤维化分级成正相关,MMP-l与纤维化分级成负相关(r=-0.465,P<0.01),肝纤维化与血清PⅢP、HA显著正相关;61例纤维化程度及计分无显著增加,血清ⅣC、LN、PⅢP、HA无明显变化,血清TGF-β、MMP-1、TIMP-1较低且变化不明显(P>0.05);19例患者炎症活动度及纤维化程度计分增加,TGF-β、TIMP-1、TIMP-1/MMP-1比值较高(P<0.01),且升高显著(P<0.01),MMP-l无显著差别,变化也不明显。结论:血清TGF-β、TIMP-1、MMP-1、TIMP-1/MMP-1水平及动态变化可作评估肝纤维化程度及发展指标。更多还原

【Abstract】 Objective:To investigate the relationship of serum transforming growth factor-β,matrix metalloproteinases-1 and tissue inhibitors of metalloproteinase-1 with the severity of liver fibrosis.Methods:A total of 80 patients with HBV related liver fibrosis and early fibrosis were enrolled from one hospital.Serum fibrosis markers including hyaluronic acid(HA),IV collagen(IV-C),aminoterminal propeptide of type Ⅲ procollagen(PⅢP),laminin(LN),matrix metalloproteinases-1(MMP-1)and tissue inhibitors of metalloproteinase-1(TIMP)-1 and transforming growth factor-β(TGF-β) Levels were determined.liver fibrosis were assessed according to a modified Scheuer and Chevallier’sscoring system.Results:In the patients of different stages of liver fibrosis,serum TGF-β,TIMP-1 and TIMP-1/MMP-1 were different(P<0.01) and serum MMP-1 was not remarkably different.Serum TGF-β(r=0.745,P<0.01),TIMP-l(r=0.623,P<0.01) and TIMP-1/MMP-l(r=0.336,P<0.01) were correlated positively with the degree of hepatic fibrosis,whereas serum MMP-1(r=-0.465,P<0.01) was correlated negatively.In The patients without remarkably increasing hepatic fibrosis degree,serum TGF-β,TIMP-1/MMP-1 and TIMP-1 were lower and had not increased.Conclusion:Serum TGF-β,TIMP-1 and TIMP-1/MMP-1 can be used as indices of evaluating hepatic fibrosis degree and growth.更多还原