【摘要】 目的:探讨乙型肝炎病毒DNA含量与乙型肝炎病毒血清标志物(HBV-M)之间的关系,为临床诊断及治疗提供有价值的依据。方法:用ELISA检测HBV-M,荧光定量聚合酶链反应(FQ-PCR)检测HBV-DNA的含量。结果:HBsAg、HBeAg、抗HBc阳性组;HBsAg、HBeAg阳性组;HBsAg,抗HBC阳性组;HBsAg、抗HBe、抗HBc阳性组;抗HBs、抗HBe、抗HBc阳性组及抗HBc阳性组中,HBV-DNA阳性率分别为:96.4%、98.0%、73.8%、51.2%、16.4%及7.4%。结论:PCR检测HBV-DNA含量与HBV-M同时测定,更有利于乙型肝炎的正确诊断,疗效判断及预后观察。更多还原

【Abstract】 Objective:To investigate the relationship between the serum HBV marker(HBV-M) and the content of HBV-DNA,in order to provide the valuable evidence for clinical diagnosis and treatment.Methods:The serum HBV marker and the content of HBV-DNA were detected with ELISA and fluorescent quantitative PCR method respectively.Results:In the six common types of serum marker(1.HBsAg,HBeAg and HBcAb were positive;2.HBsAg and HBeAg were positive;3.HBsAg and HBcAg were positive;4.HBsAg,HBeAb and HBcAb were positive;5.HBsAb,HBeAb and HBcAb were positive,6.only the HBcAb was positive),the positive rate of HBV-DNA was 96.4%,73.8%,16.4%,98.0%,51.2%,7.4% respectively.Conclusion:In order to make an accurate diagnosis,observe the curative effect and judge the prognosis,the tests of the serum HBV marker and the content of HBV-DNA should be useful at the same time.更多还原