【摘要】 本研究以两种灭活痘病毒属病毒(vaccinia virus,7.0×10~6 PFU和cowpox virus,9.2×10~7 PFU)为免疫原,分别考察不同剂量下(抗原浓度相差约100倍)实验鸡的免疫效果和相应卵黄抗体效价(免疫荧光法和抗体中和效价)。我们的研究结果,结合文献研究显示,抗原剂量对于特异性卵黄抗体的产生和表达水平具有重要影响。提高免疫原的浓度通常有助于提高抗体效价。但由于不同抗原的免疫原性差异,也有可能导致免疫抑制。针对不同免疫原似乎存在最佳免疫剂量范围。更多还原

【Abstract】 Dose-dependent IgY antibody response to different amounts of orthopox virus (OPV) antigen has been studied in immunized chickens for two different OPV strains (vaccinia virus,7.0×10~6 PFU and cowpox virus,9.2×10~7PFU).The antibody responses to different immunizations were tested and compared by indirect immunofluorescence antibody test.Our results,together with the literature,show that the antigen dose used for immunization plays an important role for the production of specific Abs.An increase in antigen concentration may achieve higher Ab titers but,dependent on the immunogenicity of OPV antigen,it can also lead to an immune depression.However,in this study we found that OPV played a positive correlation between antigen concentration and Ab-titer.更多还原