【摘要】 目的建立清肺抑火片的质量标准。方法采用薄层色谱法对清肺抑火片处方中苦参、黄柏、前胡进行了定性鉴别;采用高效液相色谱法测定处方中黄芩苷的含量。采用ODS色谱柱;流动相:甲醇-水-磷酸(47∶53∶0.2);流速:1mL/min;柱温25℃;检测波长:280nm。结果本品定性鉴别薄层色谱特征明显,专属性强;黄芩苷的线性范围为0.163～0.975μg,r=0.9999,平均回收率97.43%,RSD=1.97%(n=6)。结论该方法重现性好,简单,可有效控制清肺抑火片的质量。更多还原

【Abstract】 Objective To establilsh the quality standard for Qingfei Yihuo tablet. Methods TLC was performed to identify Sophora flavescens Ait, Peucedani and Cortex Phellodendri. HPLC was used to determine Baicalin. ODS column was used. The mobile phase consisted of methanol-water-phosphoric acid (47∶53∶0.2). The flow rate was 1.0 mL/min and column temperature was at 25 ℃. The detecting wave length was at 280 nm. Results The characteristic for identification by TLC was distinct and hightly specific. Baicalin could be determined by HPLC. The linearity of Baicalin was good in the range of 0.163～0.975 μg (r=0.999 9). The average recovery of Baicalin was 97.43% and RSD was 1.97% (n=6). Conclusion The methods of identification and quantification is simple and reproducible. It can be used effectively for the quality control of Qingfei Yihuo table.更多还原