【摘要】 目的探讨逆针关元穴对自然更年期大鼠血清、子宫的细胞因子白细胞介素-2(IL-2)、肿瘤坏死因子(TNF)、转化生长因子(TGF)和脾脏白细胞介素-2受体mRNA(IL-2R mRNA)的影响及其机制。方法将112只已经通过阴道涂片筛查的10月龄SD雌性大鼠随机分为7组,另3.5月龄1组16只。采用放射免疫、原位杂交等方法,观察10月龄逆针关元穴(2次/周,连续8周)对随后12、14、16月龄大鼠血清、子宫的细胞因子IL-2、TNF、TGF和脾脏IL-2R mRNA的影响。结果与自然年轻组相比,模型对照各组血清和子宫IL-2、TGF及子宫TNF均下降,血清TNF水平呈先降后升的波动性变化,脾脏IL-2R mRNA表达水平下降;而预先介入逆针疗法后,与自然同月龄组相比,逆针各组血清和子宫IL-2、TGF及子宫TNF上升,其中逆针14、16月龄组血清TGF,逆针12、16月龄组子宫IL-2,逆针12月龄组子宫TNF和逆针12、16月龄组子宫TGF均明显提高(P<0.05,P<0.01,P<0.01,P<0.05,P<0.01,P<0.05,P<0.05)。血清TNF水平与模型对照组呈相反的波动性变化,其中逆针12、14月龄组差异显著(均P<0.05)。脾脏IL-2R mRNA上调,其中逆针14月龄差异显著(P<0.05)。结论逆针关元穴可以调节随后更年期大鼠血清和子宫的细胞因子IL-2、TNF、TGF水平和脾脏IL-2R mRNA的表达,这可能是该疗法改善更年期免疫功能的机制之一。更多还原

【Abstract】 Objective To observe the effect on IL-2,TNF,TGF in uterus and serum,and IL-2RmRNA in spleen of menopausal rats with preventive accupuncture on Guanyuan point,and analysis the mechanism.Methods 112 ten month-aged SD female rats examined by vaginal smear were chosen and divided into seven groups.And another 16 three point five month-aged SD female rats were another group.Radio-immunity and hybridiztion in situ methods were applied to observe the effect on IL-2,TNF,TGF in uterus and serum and IL-2R mRNA in spleen of 12,14 and 16 month-aged menopausal rats with preventive accupuncture on Guanyuan point(twice a week,eight weeks continued) in 10th month.Results Compared with the normal group,IL-2,TGF in uterus and serum and TNF in uterus of menopausal rats reduced,TNF in serum came down in the 12th month and went up in 14th and 16th month,IL-2R mRNA in spleen decreased.Compared with the same-aged model group,IL-2,TGF of serum and uterus and TNF in uterus increased.TGF in serum of 14 and 16 month-aged group,IL-2 in uterus of 12 and 16 month-aged group,TNF in uterus of 12 month-aged group and TGF in uterus of 12 and 16 month-aged group rised significantly(P<0.05 or P <0.01).TNF in serum presented a converse wave-like change and it increased apparently of the 12 and 14 month-aged group(P<0.05).IL-2R mRNA increased with preventive acupuncture and it went up distinctly in the 14th month(P<0.05).Conclusion Preventive accupuncture on Guanyuan point can improve the immunologic function of menopausal rats,which is probably related to the dominating of IL-2,TNF and TGF in serum and uterus,and IL-2R mRNA in spleen.更多还原