【摘要】 目的探讨慢性氧化应激诱导动脉粥样硬化(AS)对蛋白酶体26S及20S活性的影响,以及与泛素/泛素蛋白结合物(Ub/UPC)表达之间的关系。方法新西兰雄性兔30只,随机分为正常饮食组(N)和高胆固醇饮食组(H),共饲养12周。检测26S及20S的活性,观察腹主动脉的病理变化、Ub/UPC的表达及氧化应激等指标。结果成功诱导了AS模型,H组的20S的活性比N组明显增高(P<0.01),而26S的活性两组间差异无统计学意义(P>0.05),Ub/UPC在H组的表达比N组明显增强。结论慢性氧化应激状态下,20S的活性明显增强,Ub/UPC大量聚集,可能在AS的病理变化过程中起着重要的作用。更多还原

【Abstract】 Objective To investigate correlation of proteasome 26S and 20S alteration in atherosclerosis and the accumulation of ubiquitin/ubiquitin-conjugates induced by chronic oxidized stress.Methods 30 New Zealand rabbits were randomized into normal diet (N) or therogenic diet (H) for 12 weeks.Proteasome activity,pathologic changes and ubiquitin/ubiquitin-conjugates(ub/upc) were investigated by Western blotting or immuno-chemistry.Results Atherosclerosis models were successfully induced by chronic oxidative stress.20S activity was significantly higher in H vs N(P<0.01),no significant difference of 26S activity was noted between two groups(P> 0.05).ub/upc was significantly increased H vs N.Conclusion Excessive activity of 20S and accumulation of ub/ upc may play an active role in the pathogenesis of AS induced by chronic oxidadive stress.更多还原