【摘要】 目的观察不同压力对巨噬细胞ABCA1mRNA及蛋白表达的影响。方法将THP-1源巨噬细胞依照不同压力分为对照组、60组、80组、100组、120组、140组、160组和180组,分别在大气压下,60mmHg、80mmHg、100mmHg、120mmHg、140mmHg、160mmHg和180mmHg压力下继续培养48h。观察巨噬细胞ABCA1mRNA及蛋白表达。结果60组、80组、100组、120组ABCA1mRNA分别为1.11±0.08、1.31±0.04、1.53±0.11和1.15±0.07,较对照组(0.91±0.10)明显增加(P<0.05或P<0.01),以100组增加最显著;140组、160组和180组ABCA1mRNA分别为0.75±0.06,0.46±0.08及0.35±0.05,较对照组明显减少(P<0.01)。ABCA1蛋白表达与mRNA情况相似:60组(1.09±0.06),80组(1.12±0.09),100组(1.41±0.06),120组(1.11±0.06)较对照组(1.00±0.00)明显增加(P<0.05或P<0.01),而140组(0.78±0.07)、160组(0.49±0.09)和180组(0.47±0.10)较对照组明显减少(P<0.01)。结论压力在80～120mmHg范围时,压力升高促进了巨噬细胞ABCA1mRNA及蛋白表达,压力>120mmHg后,随着压力的增高,巨噬细胞ABCA1mRNA及蛋白表达反而减少。更多还原

【Abstract】 Objective To investigated the effects of different high hydrostatic pressure on the expression of ABCA1 (mRNA, protein) in THP-1 macrophages. Methods According to the cultured pressure,they were divided into eight groups:control group(0 mm Hg),60 group(60 mm Hg),80 group (80 mm Hg),100 group (100 mm Hg),120 group (120 mm Hg),140 group (140 mm Hg),160 group (160 mm Hg) and 180 group(180 mm Hg).We determined the changes of ABCA1 mRNA and protein by reverse trancriptase polymerase chain reaction(RT-PCR) and Westernblot. Results Comparing with control group(0.91±0.10 for mRNA,1.00±0.00 for the protein),the expression level of ABCA1 mRNA and protein in THP-1 macrophages were significantly higher(P<0.05 or 0.01) in 60 group(1.11±0.08,1.09±0.06),80 group(1.31±0.04,1.12±0.09),100 group(1.53±0.11,1.41±0.06),120 group (1.15±0.07,1.11±0.06) but were significantly lower in 140 group(0.75±0.06, 0.78±0.07),160 (0.46±0.08,0.49±0.09)group and 180 group(0.35±0.05,0.47±0.10). Conclusion From 80 mm Hg to 120 mm Hg,the pressure increased markedly the expression of ABCA1(mRNA,protein) in THP-1 macrophages,but the pressure decreased markedly the expression of ABCA1(mRNA,protein) in THP-1 macrophages above 140 mm Hg.更多还原