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直接降解木质素的漆酶/木聚糖酶体系的合成

Synthesis of Laccase/Xylansae System to Directly Degrade Lignin

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【作者】 叶汉玲王玉秀严春华尤纪雪

【Author】 YE Han-ling1,2,WANG Yu-xiou1,2,YAN Chun-hua1,2,YOU Ji-xue1,2 (1. Collage of Chemical Engineering,Nanjing Forestry University; 2. Jiangsu Provincial Key Laboratory of Pulp and Paper Science and Technology,Nanjing Forestry University,Nanjing 210037,China)

【机构】 南京林业大学化学工程学院南京林业大学江苏省制浆造纸科学与技术重点实验室

【摘要】 对一株高产漆酶及伴有木聚糖酶和少量纤维素酶的菌株,用不同的碳源和氮源对其调控培养,合成漆酶/木聚糖酶体系。实验结果表明,最佳的碳源是可溶性淀粉,用它作碳源,漆酶活性高达730IU/mL,木聚糖酶活性是4.49IU/mL,纤维素酶活性只有0.23IU/mL;最佳的氮源是蛋白胨,用其作氮源,合成漆酶活性可达812IU/mL,木聚糖酶活性是4.68IU/mL,纤维素酶活只有0.09IU/mL。

【Abstract】 A strain with high production laccase,which is accompanied by the xylanase and few cellulase,is regulated with the different carbon and nitrogen source to synthesize laccase/xylanase system. The experimental result obtains the best carbon source,which is the soluble starch. Using it as a carbon source,laccase activity reaches as high as 730 IU/mL,xylanase activity is 4.49 IU/mL,and cellulase activity is 0.23 IU/mL,similarly,protein peptone is the best nitrogen source,using it as the nitrogen source,laccase activity may reach 812 IU/mL,xylanse activity is 4.68 IU/mL,and cellulase activity is only 0.09 IU/mL.

【关键词】 漆酶/木聚糖酶体系酶活木质素
【Key words】 laccase/xylanase systemenzyme activitylignin
【基金】 国家自然科学基金资助项目(30671654)研究课题之一
  • 【文献出处】 纤维素科学与技术 ,Journal of Cellulose Science and Technology , 编辑部邮箱 ,2008年03期
  • 【分类号】Q814
  • 【被引频次】4
  • 【下载频次】329
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