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水分胁迫下甘蔗根系蛋白质和核酸对外源乙烯的响应

Response of protein and nucleic acid in sugarcane root tip to extraneous ethephon under water stress

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【作者】 王威豪; 罗永明; 梁强; 叶燕萍; 李杨瑞;

【Author】 WANG Wei-hao1,2,LUO Yong-ming1,4,LIANG Qiang1,YE Yan-ping1*,LI Yang-rui1,3(1.Agricultural College,Guangxi University,Guangxi Nanning 530005,China;2.Rice Research Institute,Guangxi Academy of Agricultural Sciences,Guangxi Nanning 530007,China;3.Guangxi Academy of Agricultural Sciences,Nanning Guangxi 530007,China;4.Guangxi Agricultural Vocation-technical College,Guangxi Nanning 530007,China)

【机构】 广西大学农学院; 广西农业科学院水稻研究所; 广西农业职业技术学院; 广西农业科学院;

【摘要】 采用3种浓度乙烯利溶液(0、100、200 mg/L)分别对甘蔗新台糖22号(ROC22)、桂糖17号(GT17)和新台糖10号(ROC10)在下种前进行10 m in浸种处理,测定根系中的蛋白质含量、蛋白酶活性、核酸含量及土壤含水量的变化。结果发现,在水分胁迫条件下,100、200 mg/L乙烯利处理的蔗根均有较高的蛋白质含量和总核酸含量,GT17和ROC10的蔗根有较低的蛋白酶活性;100、200 mg/L乙烯利处理对ROC22和ROC10蔗根的RNA/DNA值有明显的促进效应,其效应以100 mg/L处理大于200 mg/L处理。试验表明乙烯利浸种可提高甘蔗品种的抗旱性。

【Abstract】 The seed canes of three sugarcane varieties i.e.ROC22,GT17 and ROC10 were soaked in ethephon solutions at 0,100,200 mg/L,for 10 min before planting.The protein content,activity of protease,nucleic acid content in roots and the soil moisture content were measured.The results showed that 100 and 200 mg/L ethephon treatments increased the protein and nucleic acid contents in the root of sugarcane,and decreased the activity of protease in the root of GT17 and ROC10 100 and 200 mg/L ethephon treatments had higher ratio of RNA to DNA in the roots of ROC22 and ROC10 than that of GT17 under water stress,and the best effect was found in the 100 mg/L ethephon treatment.The results suggested that the ethephon treatment could increase the drought resistance of sugarcane.

【关键词】 水分胁迫; 乙烯利; 甘蔗; 蛋白质; 核酸;
【Key words】 water stress; ethephon; sugarcane; protein; nucleic acid;
【基金】 广西大学博士科研启动基金项目(X071040)
  • 【文献出处】 西南农业学报 ,Southwest China Journal of Agricultural Sciences , 编辑部邮箱 ,2008年06期
  • 【分类号】S566.1
  • 【被引频次】7
  • 【下载频次】201
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