【摘要】 研究了马歇尔红(Marshall red R,MR)与牛血清蛋白(BSA)作用的共振光散射(RLS)光谱特征.考察了各种影响因素并计算出了BSA与MR的结合比(质量比)为3.2.研究表明,在优化条件下,体系的RLS强度与蛋白质浓度在一定范围内具有良好的线性关系,据此建立了一种蛋白质测定的新方法.在最佳实验条件下,BSA的线性范围是0.03~6.0μg/mL,最低检出限为0.9 ng/mL.该方法用于合成样品及尿液的测定,结果满意.更多还原

【Abstract】 The interaction of bovine serum albumin(BSA) with MR was studied by resonance light scattering(RLS)technique and UV-vis spectra.At pH 2.36,a new method for the determination of proteins based on the enhancement of RLS was developed.Under the optimum conditions,the linear ranges of the calibration graphs were 0.03-6.0 μg/mL for proteins and the corresponding determination limit was 0.9 ng/mL.Satisfactory results were obtained when this method was applied in the analysis of samples of foreign substances and urine.更多还原