【摘要】 目的:观察缺氧性肺动脉高压大鼠循环内皮细胞(CEC)形态学变化。方法:50只大鼠随机分成5组,A组:正常组;B组:缺氧2 w组;C组:缺氧2 w叠加FeCl3组;D组:缺氧4 w组;E组:缺氧4 w叠加FeCl3组。缺氧叠加静注FeCl3模型在每周第3、6 d的缺氧后尾静脉注射0.6%FeCl3。各组分别取血运用多功能显微镜观察CEC的形态学特点。结果:缺氧性肺动脉高压时右室压、肺动脉平均压较正常组升高(P<0.01)。正常组可见少量散在呈不规则多边形的无核、皱缩、折叠或扭曲状CEC;缺氧组CEC数量增加(P<0.01),多有核,胞浆丰富,可见3个以上内皮细胞成片状脱落;缺氧叠加FeCl3组内皮损伤较单纯缺氧组加重。结论:缺氧可致CEC数量增多,缺氧越明显,成片脱落CEC越多,炎症可加重内皮损伤。更多还原

【Abstract】 Objective:To observe the morphological changes of circulating endothelial cells(CEC) in rats with hypoxic pulmonary hypertension.Methods:50 male SD rats were randomly divided into 5 groups: group A(normal group),group B(hypoxia for 2 w),group C(hypoxia for 2 w with FeCl3 intravenous injection),group D(hypoxia for 4 w) and group E(hypoxia for 4 w with FeCl3 intravenous injection).Rats in group C and E were injected 0.6% FeCl3 through caudal vein at the 3rd,6th day every week.Then,blood samples of all groups were obtained to observe the morphological changes of circulating endothelial cells under Multi-functional microscopy.Results:There were more CEC in hypoxia groups than those in normal group(P<0.01).These CEC had rich plasma and nucleus.Some slices consisted of over 3 such cells were found to peel off into the blood.The CEC numbers were markedly increased in hypoxia groups with FeCl3 intravenous injection.Conclusion:Hypoxia can increase CEC numbers.The more markedly the hypoxia is,the more cells peel into the blood.Inflammation can aggravate the endothelial cell injury.更多还原