【摘要】 目的:观察大鼠脑组织缺血再灌注后脑组织损伤变化及丹参的作用。方法:将42只SD大鼠随机分为正常组6只、缺血再灌注组(缺血组)18只和丹参治疗组(丹参组)18只,血管阻断法制作大鼠左侧脑缺血再灌注模型。丹参组于再灌注开始时腹腔注射丹参注射液(5 ml/kg),而缺血组注入等量生理盐水。观察时点为脑缺血再灌注后12 h、24 h和48 h,观察大鼠脑组织病理变化,检测脑组织含水率及细胞凋亡情况(TUNEL法),检测脑组织丙二醛(malondialdehyde,MDA)、谷氨酸(glutamate,GLU)和γ氨基丁酸(gamm-aminobutyric,GABA)含量变化。结果:与正常组比较,缺血组脑组织含水率、凋亡细胞数、MDA水平和GLU含量显著升高,GABA含量显著降低。与缺血组同时点比较,丹参组脑组织病理变化较轻,脑组织含水率、凋亡细胞数、MDA水平和GLU含量显著降低,GABA含量显著升高。结论:血管阻断法能造成显著的大鼠脑缺血再灌注损伤,丹参能有效降低其损伤效应。更多还原

【Abstract】 Objective:To observe the changes of brain tissue after ischemia reperfusion injury and to investigate the protective effects of salvia miltiorrhiza(SM).Methods:42 SD rats were divided into normal group(n=6),ischemia reperfusion injury group(control group,n=18) and SM group(n=18) at random.The model of ischemia reperfusion injury was made by occlusion of middle cerebral artery.SM injection was given to the rats in SM group through peritoneal injection at the dose of 5 ml/kg.The normal saline was given to the rats of the control group.The pathological changes,water ratio and cell apoptosis of these rats’ brain tissue were observed after ischemia reperfusion injury in 12 h,24 h and 48 h.At the same time,the contents of MDA,glutamate(GLU) and gamma-aminobutyric(GABA) in the brain of the rats were detected.Results:Compared with the normal group,the water ratio,number of apoptotic cell,contents of MDA and GLU of the control group increased significantly and the content of GABA decreased significantly.The water ratio,number of apoptotic cell,contents of MDA and GLU of SM group were lower and GABA was higher significantly than those of the control group.Conclusion:SM can alleviate significantly the ischemia reperfusion injury of brain in rats.更多还原