【摘要】 根据鸡的Myostatin基因序列设计引物,从鹅的基因组DNA中扩增到了Myostatin基因的5′-调控区,克隆并测序后获得了1.3 kb片段序列。与其他物种的序列比较发现,鹅Myostatin基因的该调控序列与鸭同源性为94.8%,与鸡同源性为69.2%,而与小鼠仅为14.3%。通过生物信息学方法,分析确定了其启动子和转录起始位点,发现在转录起始位点上游-34 bp处存在1个TATA盒,-77 bp处存在1个CAAT盒。还发现在该片段中包含5个E框、2个MEF2结合位点、1个MSX2盒和1个MTATA盒等肌肉特异性转录因子结合位点。采集鹅肝脏、胸肌、腿肌、心脏、肺、肠、肾和脑等8种组织并提取总RNA,用Northern blot方法检测了Myostatin基因mR-NA在8种组织中的表达情况,发现其只在胸肌和腿肌中表达,而在其他几种组织中没有检测到表达信号。更多还原

【Abstract】 The primers of 5′-regulatory region were designed according to chicken Myostatin gene.The fragment was amplified from goose genomic DNA,and cloned into pGEMT-Easy Vector and sequenced.A 1.3 kb long fragment of 5′-regulatory region of goose Myostatin gene,which includes the putative initial transcriptional site,was acquired.The sequence homology analyses showed that the goose Myostatin 5′-regulatory region shared 94.8% with duck,69.2% with chicken and 14.3% with mouse.Sequence analyses showed that there are a TATA box at-34 bp and a CCAAT box at-77 bp upstream of the putative transcriptional site.In addition,some muscle specific transcriptional factor binding sites including 5 E-boxes,2 MEF2 binding sites,a MSX2 box and a muscle TATA box were determined.The Northern blot results in goose showed that Myostatin mRNA only expressed in breast muscle and thigh muscle and were not detectable in liver,heart,intestine,kidney,brain and lung.更多还原