【摘要】 模拟磺胺母核结构合成了3种人工免疫原,免疫新西兰白兔,用间接竞争ELISA方法监测免疫效果。选择抑制效果较好的2组多抗血清建立磺胺类药的ELISA检测方法,绘制竞争标准曲线,并进行添加回收试验。结果表明:A组多克隆抗体对6种常用磺胺药SMD、SMM、SMZ、SDM、SDM′、SD的IC10分别为0.010、0.046、0.010、0.100、0.067、0.019μg/mL,低于最高残留限量(0.10μg/mL);C组多克隆抗体对SMD、SMM、SMZ、SDM、SQ、SD、磺胺氯哒嗪、磺胺氯吡嗪的IC10分别为0.0091、0.051、0.058、0.088、0.020、0.010、0.014、0.011μg/mL,亦低于最高残留限量。2组多克隆抗体对磺胺药的回收率为54.5%~111.8%和54.2%~130.3%,变异系数为3.6%~10.9%和3.7%~5.9%。更多还原

【Abstract】 New Zealand White rabbits were immunized with three artificial immunizing antigens that modeling the common structure of sulfonamides,and the immune effect were detected by ELISA.A new Ci-ELISA method against sulfonamides and standard curves were established using two polyclonal antisera because of their good inhibitory effect.The IC10 of polyclonal antisera from group A against SMD,SMM,SMZ,SDM,SDM′ and SD was 0.010,0.046,0.010,0.067,0.019 μg/mL respectively,which was less than the MRLs(0.1 μg/mL).The IC10 of polyclonal antisera from group C against SMD,SMM,SMZ,SDM,SQ,SD,sulfachloropyridazine and sulfachaoropyrazine were 0.009 1,0.051,0.058,0.088,0.020,0.010,0.014,0.011 μg/mL respectively,less than the MRLs.The recoveries of two polyclonal antibodies ranged 54.5%-111.8%,54.2%-130.3% with CV of 3.6%-10.9% and 3.7%-5.9%,respectively.更多还原