【摘要】 应用PCR-RFLP技术检测猪磷脂转移蛋白(PLTP)基因内含子4多态性。结果表明:凉伞猪、龙潭猪群体中PLTP基因内含子4经HaeⅢ酶切后均有A和B 2个等位基因存在。利用最小二乘分析研究该多态位点对初生质量、45日龄质量、60日龄质量、4月龄质量、6月龄体质量、6月龄体高、6月龄体长、6月龄胸围等生长性状的影响。结果显示:aa与AA基因型个体间在4月龄体质量上存在极显著差异(P<0.01),同样在6月龄体高上存在显著差异(P<0.05),Aa基因型个体在初生质量上显著高于AA基因型个体(P<0.05)。由此可知,猪PLTP基因不同基因型对生长性状有着重要的影响,是猪育种应用中的一个潜在的遗传标记。更多还原

【Abstract】 The polymorphism of intron 4 in phospholipid transfer protein(PLTP) gene was detected by PCR-RFLP.A Hae Ⅲ RFLP was detected in intron 4 and the polymorphic locus is common in all detected groups.Associations between the founded Hae Ⅲ RFLP in intron 4 and birth weight,body weight at 45 days,body weight at 60 days,body weight at 4 months,body weight at 6 months,body height at 6 months,body length at 6 months and circumference at 6 months were tested by least square analysis.No significant difference between Hae Ⅲ RFLP and body weight at 45 days,body weight at 60 days,body weight at 6 months,body length at 6 months and circumference at 6 months were founded in this study.The results showed that the body weight at 4 months with aa genotype was higher than that with AA genotype(P<0.01),the body height at 6 months with aa genotype was higher than that with AA genotype(P<0.05),the birth weight with Aa genotype was higher than that with AA genotype(P<0.05).Therefore,it could be seen that porcine PLTP genotypes had significant influence on growth traits,this SNP might be considered as a potential genetic marker for selection.更多还原