【摘要】 为了探索硒对妊娠早期子宫局部细胞因子IL-2I、L-4、TGF-β1及TGF-β2的调节作用,将妊娠山羊分为试验Ⅰ、Ⅱ、Ⅲ组和对照组。Ⅰ、Ⅱ、Ⅲ组山羊分别以0.3、0.5和0.7 mg/kg剂量的硒灌服亚硒酸钠溶液,对照组灌服1mL/kg的蒸馏水,每周1次,连续3周。用ELISA试剂盒检测子宫局部细胞因子IL-2、IL-4、TGF-β1及TGF-β2的动态变化以观察硒对其分泌的调节作用。结果表明:(1)妊娠早期(15~19 d)山羊两侧子宫角中IL-2I、L-4、TGF-β1及TGF-β2水平无显著差异。妊娠早期山羊两侧子宫角的子宫液IL-2水平在15~18 d呈上升趋势,19 d时趋于下降;IL-4水平一直呈上升趋势;TGF-β1水平较高,呈不规律变化;TGF-β2呈现先升高、后趋于平稳的趋势。(2)灌服亚硒酸钠溶液,可以显著降低妊娠16~18 d子宫液IL-2的水平(P<0.05或P<0.01);同时可显著升高IL-4的水平(P<0.05或P<0.01),且呈一定的剂量依赖关系。(3)0.5和0.7 mg/kg剂量组显著升高妊娠16~18 d子宫液TGF-β1水平(P<0.05或P<0.01);同时对妊娠16~17 d子宫液TGF-β2水平也有显著的升高作用(P<0.05或P<0.01),但对18 d时的TGF-β2影响不显著。试验表明,硒对妊娠早期山羊子宫局部IL-2I、L-4、TGF-β1及TGF-β2的分泌具有一定的调节作用。更多还原

【Abstract】 In order to explore the regulation of the local-uterine cytokine IL-2,IL-4,TGF-β1 and TGF-β2 by selenium(Se) during early gestation of goats,the pregnant goats were grouped into experimental groups Ⅰ,Ⅱ,Ⅲ and control group.Group Ⅰ,Ⅱand Ⅲ were treated with sodium selenite respectively at doses of 0.3,0.5 and 0.7 mg(Se)/kg by oral administration weekly for three times and control group was similarly treated with distilled water.Uterine samples were collected to determine the content of IL-2,IL-4,TGF-β1 and TGF-β2 by ELISA.The results showed that(1)There were no differences at the levels of all the tested cytokine between two uterine horns of each goat during early gestation.The IL-2 levels in fluid of two uterine horns increased during 15-18 d after pregnancy and began to decrease on day 19 after pregnancy,and IL-4 remained in a continuously high level during 15-19 d after pregnancy.The TGF-β1 had irregular high level in the same period and TGF-β2 increased primely and then remained at a high level.(2) Administration of 0.3,0.5 and 0.7 mg/kg selenium could significantly decrease the content of IL-2 in uterine fluid during 16-18 d after pregnancy and significantly increase the level of IL-4 in uterine fluid in a dose-dependent manner.(3)Goats of 0.5 and 0.7 mg/kg groups showed significantly high level of TGF-β1 in uterine fluid during 16-18 d after pregnancy,and presented high level of TGF-β2 in uterine fluid during 16-17 d after pregnancy but no significant effect on TGF-β2 secretion on day 18 after pregnancy.These results indicated that selenium has a regulatory function on secretion of the local-uterine IL-2,IL-4,TGF-β1 and TGF-β2 during early pregnancy in goats.更多还原