【摘要】 克隆了猪PGAM2基因的cDNA序列,并利用生物信息学方法进行分析。所得cDNA序列全长913 bp,包含1个完整的开放阅读框,编码253个氨基酸。序列分析表明,该基因预测编码蛋白与已报道的狗(97.6%)、人(96%)、黑猩猩(96%)、大鼠(94.5)和小鼠(94.1%)等物种的PGAM2蛋白高度同源其在N端和C端分别存在1个ATHR和QGKA模体,在14~15AA处存在1个信号肽切割位点,含有1个典型的碳水化合物运输和代谢(GPMA)保守结构域。该基因在猪胚胎骨骼肌发育过程中差异表达,且在通城猪和长白猪中呈现不同表达模式。在通城猪中,PGAM2基因呈波浪式表达,即妊娠65 d时表达水平最高,且各时间点表达水平差异极显著(P<0.01)。在长白猪中呈上调表达模式,妊娠65和90 d时表达丰富,且极显著高于妊娠33 d(P<0.01)。品种间比较来看,在妊娠33和65 d时两品种表达水平相当,但妊娠90 d时长白猪中的表达水平极显著高于通城猪(P=0.006)。更多还原

【Abstract】 The cDNA sequences of porcine PGAM2 gene were cloned,being analyzed by bioinformatics method.The full-length cDNA was 913 bp with an entire open reading frame(ORF),encoding 253 amino acids.The analysis of sequence revealed that the porcine PGAM2 gene was highly homologous with that of other species,such as canis familiaris(97.6%),Homo sapiens(96%),Pan troglodytes(96%),Rattus norvegicus(94.5%) and Mus musculus(94.1%) in amino acid.The protein encoded by porcine PGAM2 gene had ’ ATHR’ and ’QGKA’ motif at N-and C-terminatus sites,respectively.There were a cut site of signal peptide sequence at 14-15 AA and a typical conserved domain ’carbohydrate transport and metabolism(GPMA)’.The PGAM2 gene was differently expressed during fetus skeletal muscle development with different expression pattern between Tongcheng and Landrace pigs.In Tongcheng pigs,it was differently expressed with wave pattern at selected stages,and expressed most abundantly at 65 days post coitus(dpc).In Landrace,PGAM2 gene was expressed with ’up-regulation’ expression pattern and expressed abundantly at 65 and 90 dpc more than that at 33 dpc.There was higher expression in Landrace pigs than that in Tongcheng pigs at 90 dpc,but no significant difference at 33 and 65 dpc between the two breeds.更多还原